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21.
Thresholds,sensitive stages and genetic variability of finger millet to high temperature stress 下载免费PDF全文
R. A. Opole P. V. V. Prasad M. Djanaguiraman K. Vimala M. B. Kirkham H. D. Upadhyaya 《Journal of Agronomy and Crop Science》2018,204(5):477-492
Finger millet [Eleusine coracana (L.) Gaertn.] is an important coarse cereal crop grown in the arid and semi‐arid regions and often experiences high temperature (HT) stress. The objectives of this research were (i) to quantify effects of season‐long HT stress on physiological and yield traits, (ii) to identify the developmental stages most sensitive to HT stress and (iii) to quantify the genetic variability for HT stress tolerance in finger millet. Research was conducted in controlled environment conditions. HT stress decreased the chlorophyll index, photosystem II activity, grain yield and harvest index. Maximum decrease in number of seeds per panicle and grain yield per plant was observed when stress was imposed during booting, panicle emergence or flowering stages. Maximum genotypic variation was explained by panicle width and number of seeds per panicle at optimum temperature (OT) and grain yield per plant at HT and number of seeds at HT. Based on the stress response and grain yield, tolerant or susceptible genotypes were identified. Finger millet is sensitive to HT stress during reproductive stages, and there was genotypic variability among the finger millet genotypes for number of seeds per panicle and grain yield under HT, which can be exploited to enhance stress tolerance. 相似文献
22.
Variation in prey selection and foraging success associated with early‐life ontogeny and habitat use of American paddlefish (Polyodon spathula) 下载免费PDF全文
William D. Hintz Nicolle K. MacVey Allison M. Asher Anthony P. Porreca James E. Garvey 《Ecology of Freshwater Fish》2017,26(2):181-189
Many fishes are planktivorous during early life and switch to piscivory or consume larger food items as ontogeny progresses. In contrast, paddlefish start as particulate feeders and later become filter feeders. Few studies have identified food items essential for paddlefish growth, survival and recruitment surrounding this ontogenetic diet shift. The objectives of this study were to investigate (i) prey types consumed by paddlefish, (ii) variability in prey selection surrounding an ontogenetic diet shift and (iii) whether habitat affected paddlefish prey selection or foraging success. We analysed gut contents of 189 wild age‐0 paddlefish from the middle Mississippi River (MMR) and also conducted a laboratory experiment to address these objectives. We found that paddlefish primarily foraged on benthic macroinvertebrates in the MMR, which differed from previous studies in lentic systems, suggesting young paddlefish prey selection may be labile depending on habitat (i.e., lotic versus lentic). Dominant prey of wild‐caught and experimental age‐0 paddlefish were caddisfly larvae (Trichoptera), followed by hemipterans and amphipods. We found little evidence that habitat attributes affected foraging success, but the most common prey items consumed were associated with wing dike habitat and the upstream and downstream tips of alluvial islands. Our experimental study revealed that if provided a mixture of organisms, age‐0 paddlefish will primarily consume macroinvertebrates while age‐1 paddlefish will mainly filter zooplankton. Overall results suggest young paddlefish prey selection can be highly variable, but also heavily reliant on a narrow group of prey resources. 相似文献
23.
Natural resistance in cottonseed to Aspergillus flavus infection has not been explored to date. A green fluorescent protein (GFP) expressing A. flavus strain was used to assess the resistance of seed from 35 cotton varieties from Gossypium arboreum, G. barbadense, and G. hirsutum. Mature cotyledons devoid of seed coat were wounded, inoculated, and assessed for innate resistance to A. flavus infection. Of the initial 35 varieties tested, we observed a range of resistance to infection in representatives of all three species. A subset of 15 representatives was further analyzed. Within this group, G. arboreum cultivar A2 186 and G. hirsutum cultivar SA 1582 were most resistant to fungal infection. The most susceptible cultivar in this group was G. hirsutum SA 1595. The remaining 12 representatives tested in the secondary screen (3 G. arboreum, 3 G. barbadense, and 6 G. hirsutum lines) exhibited intermediate resistance. We did not observe any relationship between species and resistance. Within each species, there was a range of responses to fungal infection. Future studies using this methodology to screen additional diploid and tetraploid cotton lines may enable us to identify naturally resistant germplasm that can be used to develop cotton with enhanced resistance to fungal infection. 相似文献
24.
Assessing vulnerability of New Zealand lakes to loss of conservation value from invasive fish impacts 下载免费PDF全文
- Predictions of invasion risk for seven non‐indigenous fish species, ecological impact scores for individual species, and lake conservation rankings were linked to develop Invasion Risk Impact (IRI) and Lake Vulnerability (LV) indices that help identify New Zealand lakes most at risk of loss of conservation value from potential multi‐species invasions.
- Species‐specific IRI scores (the product of predicted invasion risk and species impact) highlighted Eurasian perch (Perca fluviatilis) and the brown bullhead (Ameiurus nebulosus), as the species most likely to spread and cause ecological harm in lakes. For 3431 lakes >1 ha throughout New Zealand, total IRI tended to be highest for lowland riverine and dune lakes most of which are already colonized by multiple invasive fish species.
- The LV index indicated that lakes most at risk of loss of conservation value from invasive fish impacts were predominantly (i) in the northern half of the North Island where several uncommon lake types occur, and (ii) along the west coast of the South Island where conservation value is often greater, largely because of low catchment modification.
- The IRI and LV indices can be used to assist with setting priorities for surveillance monitoring, advocacy, and response planning targeted at preventing the establishment of invasive fish in moderate‐to‐high value lakes most susceptible to ecological impacts. Both indices can be adapted to accommodate alternative impact and conservation scoring systems, providing a flexible tool for local‐ and national‐scale assessments of lake vulnerability to fish invasion impacts.
25.
M. Bjørnevik H. Hansen B. Roth A. Foss E. Vikingstad C. Solberg A.K. Imsland 《Aquaculture Nutrition》2017,23(2):285-292
The study was designed to investigate the effect of four cycles of 5 weeks starvation followed by 10‐week refeeding compared with daily feeding under either natural photoperiod or continuous light (LL) regime on body composition and flesh quality in Atlantic cod in sea cages, northern Norway. The fish were sampled for body composition and flesh quality parameters at the start of the trial, twice at the end of a 10‐week feeding period and twice at the end of a 5‐week starvation period. There was effect of both feeding and light regime on growth, the two starving groups losing weight during starvation and regaining weight during refeeding, and the group under LL being heavier. But, the mean overall growth did not vary between groups. Starvation/refeeding regime resulted in higher slaughter yield, but no overall effect was seen on hepatosomatic index, water content, water holding capacity (WHC), muscle pH, hardness or flesh colour compared with control groups. Continuous light increased gutted weight and slaughter yield, lowered WHC and depressed maturation compared with fish under natural light regime. Increased growth rate resulted in softer fillets and lower muscle pH. 相似文献
26.
Daniel P. Harrison Michael G. Hinton Suzanne Kohin Edward M. Armstrong Stephanie Snyder Frank O'Brien Dale K. Kiefer 《Fisheries Oceanography》2017,26(3):316-335
We have developed a set of tools that operate within an aquatic geographic information system to improve the accessibility, and usability of remote‐sensed satellite and computer‐modeled oceanographic data for marine science and ecosystem‐based management. The tools form the Pelagic Habitat Analysis Module (PHAM), which can be applied as a modeling platform, an investigative aid in scientific research, or utilized as a decision support system for marine ecological management. Applications include fisheries, marine biology, physical and biological oceanography, and marine spatial management. The GIS provides a home for diverse data types and automated tools for downloading remote sensed and global circulation model data. Within the GIS environment, PHAM provides a framework for seamless interactive four‐dimensional visualization, for matching between disparate data types, for flexible statistic or mechanistic model development, and for dynamic application of user developed models for habitat, density, and probability predictions. Here we describe PHAM in the context of ecosystem‐based fisheries management, and present results from case study projects which guided development. In the first, an analysis of the purse seine fishery for tropical tuna in the eastern Pacific Ocean revealed oceanographic drivers of the catch distribution and the influence of climate‐driven circulation patterns on the location of fishing grounds. To support management of the Common Thresher Shark (Alopias vulpinus) in the California Current Ecosystem, a simple empirical habitat utilization model was developed and used to dynamically predict the seasonal range expansion of common thresher shark based on oceanographic conditions. 相似文献
27.
Anchal Dass Subhash Chandra Norman Uphoff Anil K. Choudhary Ranjan Bhattacharyya K. S. Rana 《Paddy and Water Environment》2017,15(4):745-760
Although the System of Rice Intensification (SRI) has been reported to produce higher paddy (Oryza sativa L.) yields with better-quality grains, little research has addressed the latter claim. This study investigated the interactive effects of rice cultivation methods with different irrigation schedules and plant density on the uptake and concentration of sulfur (S), zinc (Zn), iron (Fe), manganese (Mn) and copper (Cu) in the grain and straw of two rice cultivars during two rainy seasons in the northern plains of India. As the two seasons differed in amounts of rainfall, there were impacts of soil moisture differences on nutrient uptake. Plots with SRI cultivation methods enhanced the grain uptake and concentrations of S, Zn, Fe, Mn and Cu by 36, 32, 28, 32 and 63%, respectively, compared to conventional transplanting (CT). Under SRI management, the highest concentrations of S, Zn and Cu in the grain and straw occurred with irrigation intervals scheduled at 3 days after disappearance of ponded water (DADPW; 3D), whereas Fe and Mn concentrations in the grain and straw were higher with irrigation at 1 DADPW (1 D ) compared with plots under 3 D or 5 DADPW (5 D ). The higher nutrient uptakes were also manifested in higher grain yield in 1 D and 3 D plots (by 9 and 6%, respectively) compared with 5 D . Wider spacing (25 × 25 cm) compared with closer spacing (20 × 20 cm) significantly increased yield and the uptake and concentrations of all the said nutrients in the grains. When comparing the performance of two cultivars, the total uptakes of Zn, Fe, Mn and Cu in both grain and straw were significantly more in Hybrid 6444 than the improved variety Pant Dhan 4. Overall, SRI crop management compared to CT practices led to more biological fortification of rice grains with respect to S and the four micronutrients studied, giving a concomitant yield advantage of about 17% on average in this region. 相似文献
28.
M. Akhlasur Rahman Isaac Kofi Bimpong J. B. Bizimana Evangeline D. Pascual Marydee Arceta B. P. Mallikarjuna Swamy Faty Diaw M. Sazzadur Rahman R. K. Singh 《Rice》2017,10(1):47
Background
Salinity is one of the most severe and widespread abiotic stresses that affect rice production. The identification of major-effect quantitative trait loci (QTLs) for traits related to salinity tolerance and understanding of QTL × environment interactions (QEIs) can help in more precise and faster development of salinity-tolerant rice varieties through marker-assisted breeding. Recombinant inbred lines (RILs) derived from IR29/Hasawi (a novel source of salinity) were screened for salinity tolerance in the IRRI phytotron in the Philippines (E1) and in two other diverse environments in Senegal (E2) and Tanzania (E3). QTLs were mapped for traits related to salinity tolerance at the seedling stage.Results
The RILs were genotyped using 194 polymorphic SNPs (single nucleotide polymorphisms). After removing segregation distortion markers (SDM), a total of 145 and 135 SNPs were used to construct a genetic linkage map with a length of 1655 and 1662 cM, with an average marker density of 11.4 cM in E1 and 12.3 cM in E2 and E3, respectively. A total of 34 QTLs were identified on 10 chromosomes for five traits using ICIM-ADD and segregation distortion locus (SDL) mapping (IM-ADD) under salinity stress across environments. Eight major genomic regions on chromosome 1 between 170 and 175 cM (qSES1.3, qSES1.4, qSL1.2, qSL1.3, qRL1.1, qRL1.2, qFWsht1.2, qDWsht1.2), chromosome 4 at 32 cM (qSES4.1, qFWsht4.2, qDWsht4.2), chromosome 6 at 115 cM (qFWsht6.1, qDWsht6.1), chromosome 8 at 105 cM (qFWsht8.1, qDWsht8.1), and chromosome 12 at 78 cM (qFWsht12.1, qDWsht12.1) have co-localized QTLs for the multiple traits that might be governing seedling stage salinity tolerance through multiple traits in different phenotyping environments, thus suggesting these as hot spots for tolerance of salinity. Forty-nine and 30 significant pair-wise epistatic interactions were detected between QTL-linked and QTL-unlinked regions using single-environment and multi-environment analyses.Conclusions
The identification of genomic regions for salinity tolerance in the RILs showed that Hasawi possesses alleles that are novel for salinity tolerance. The common regions for the multiple QTLs across environments as co-localized regions on chromosomes 1, 4, 6, 8, and 12 could be due to linkage or pleiotropic effect, which might be helpful for multiple QTL introgression for marker-assisted breeding programs to improve the salinity tolerance of adaptive and popular but otherwise salinity-sensitive rice varieties.29.
Otolith chemistry indicates walleye movement and entrainment in a large serial reservoir system 下载免费PDF全文
Understanding fish movement in impounded river systems is important for fisheries management. Otolith chemistry was used to examine walleye Sander vitreus (Mitchill) intra‐ and inter‐reservoir movement in four Missouri River impoundments. Age‐0 individuals were reclassified with 75%–93% accuracy to known natal sites, allowing for reliable evaluation of movement patterns of age‐1 and older fish. Nearly half of walleye occupied the same location annually (i.e. site residency) between 2009 and 2010. In 2011, during the largest flood on record since 1898, downstream movement (49% of age‐1 and older fish) exceeded upstream movement (7%) and site residency (33%) across the study area. After the flood, most walleye moved downstream within reservoirs (45%) or were site residents (37%). Entrainment occurred most frequently during the flood year and was proportionally greatest in downstream reservoirs. Otolith chemistry is useful for understanding walleye movement and entrainment and is a tool for fisheries management with applications such as informing reservoir water releases, harvest regulations and habitat protection and rehabilitation within and outside the Missouri River reservoir system. 相似文献
30.
S J Monaghan S M Bergmann K D Thompson L Brown T Herath J del‐Pozo A Adams 《Journal of fish diseases》2017,40(8):1041-1054
Cyprinid herpesvirus 3 (CyHV‐3) is an alloherpesvirus, and it is the aetiological agent of koi herpesvirus disease. Although the complex morphogenic stages of the replication cycle of CyHV‐3 were shown to resemble that of other members of the Herpesvirales, detailed analysis of the sequence and timing of these events was not definitively determined. This study describes these features through a time course using cyprinid cell cultures (KF‐1 and CCB) infected with CyHV‐3 (KHV isolate, H361) and analysed by transmission electron microscopy. Rapid viral entry was noted, with high levels of intracellular virus within 1–4 h post‐infection (hpi). Intranuclear capsid assembly, paracrystalline array formation and primary envelopment of capsids occurred within 4 hpi. Between 1 and 3 days post‐infection (dpi), intracytoplasmic secondary envelopment occurred, as well as budding of infectious virions at the plasma membrane. At 5–7 dpi, the cytoplasm contained cytopathic vacuoles, enveloped virions within vesicles, and abundant non‐enveloped capsids; also there was frequent nuclear deformation. Several morphological features are suggestive of inefficient viral assembly, with production of non‐infectious particles, particularly in KF‐1 cells. The timing of this alloherpesvirus morphogenesis is similar to other members of the Herpesvirales, but there may be possible implications of using different cell lines for CyHV‐3 propagation. 相似文献